Structural Elucidation by High Energy CID-MS/MS.

Structural Elucidation of the Mechanistic

Complete structural elucidation of triacylglycerols by tandem sector mass spectrometry.

We developed a method to elucidate the complete structure of triacylglycerols by means of high-energy collisional activation tandem mass spectrometry (MS/MS). Both ESI- and FAB-produced [M + NH4]+ and [M + met.]+ ions (where met. = Li, Na, and Cs) of triacylglycerols undergo charge-remote and charge-driven fragmentations. We emphasize the study of fragment ions from ESI-produced [M + NH4]+ and ...

ISOLATION AND STRUCTURAL ELUCIDATION OF THE FIRST KNOWN C-HOMOPROTOBERBERINE

A novel C-homoprotoberberine alkaloid, Hediamine (2), has been isolated from the roots of Berberis actinucantha (Berberidaceae). Conclusive proof for the structure of hediamine was obtained by its comparison with the isomeric lactam (4), by chemical transformation, and by means of spectroscopic techniques (UV, IR, MS, NMR).

Structural Characterization of Sulfoquinovosyl, Monogalactosyl and Digalactosyl Diacylglycerols by FAB-CID-MS/MS

The glycolipids such as sulfoquinovosyl diacylglycerol (SQDG), isolated from wild-type cyanobacterium Synechocystis sp. PCC 6803, and monogalactosyl diacylglycerol (MGDG) and digalactosyl diacylglycerol (DGDG), obtained from whole wheat Ñour, have been investigated by using fast atom bombardment tandem mass spectrometry (FAB-MS/MS). Collision-induced dissociation (CID) of sodium-adduct molecula...

Glycopeptide Site Heterogeneity and Structural Diversity Determined by Combined Lectin Affinity Chromatography/IMS/CID/MS Techniques.

Glycopeptides from a tryptic digest of chicken ovomucoid were enriched using a simplified lectin affinity chromatography (LAC) platform, and characterized by high-resolution mass spectrometry (MS) as well as ion mobility spectrometry (IMS)-MS. The LAC platform effectively enriched the glycoproteome, from which a total of 117 glycopeptides containing 27 glycan forms were identified for this prot...